验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, A549 cells, rat liver tissue |
| Positive IHC detected in | human cerebellum tissue, human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22980-1-AP targets PRODH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PRODH fusion protein Ag18429 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | proline dehydrogenase (oxidase) 1 |
| Synonyms | HSPOX2, p53 induced gene 6 protein, PIG6, POX, POX2, PRODH, PRODH1, Proline oxidase, Proline oxidase 2, SCZD4, TP53I6 |
| Calculated Molecular Weight | 600 aa, 68 kDa |
| Observed Molecular Weight | 56 kDa, 66 kDa |
| GenBank Accession Number | BC118597 |
| Gene Symbol | PRODH |
| Gene ID (NCBI) | 5625 |
| RRID | AB_2879191 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43272 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRODH(Proline dehydrogenase 1, mitochondrial) is also named as PIG6, HSPOX2, PRODH1, PRODH2, POX, SCZD4, TP53I6 and belongs to the proline oxidase family. It is an oxidoreductase involved in the transfer of redox potential across the mitochondrial membrane and catalyzes the rate-limiting oxidation of proline to pyrroline- 5-carboxylate (P5C). High PRODH activity is sufficient to induce mitochondria-mediated apoptosis in the presence of proline. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1) and defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). This protein has 3 isoforms produced by alternative splicing with the molecular mass of 68 kDa, 59 kDa and 56 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRODH antibody 22980-1-AP | Download protocol |
| IHC protocol for PRODH antibody 22980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress. | ||
Cell Rep Proline restores mitochondrial function and reverses aging hallmarks in senescent cells | ||
Oxid Med Cell Longev Metabolomic Analysis of the Ameliorative Effect of Enhanced Proline Metabolism on Hypoxia-Induced Injury in Cardiomyocytes.
| ||
Amino Acids PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma. | ||
Amino Acids Activation of proline metabolism maintains ATP levels during cocaine-induced polyADP-ribosylation.
| ||
Animals (Basel) Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine. |
