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PRPF19 Polyclonal antibody

PRPF19 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15414-1-AP

Print datasheet

Synonyms

RING-type E3 ubiquitin transferase PRP19, PSO4, PRP19, Pre-mRNA-processing factor 19, hPso4

验证数据展示

  • WB analysis of mouse kidney using 15414-1-AP

    WB analysis of mouse kidney using 15414-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 15414-1-AP

    IHC staining of human stomach using 15414-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15414-1-AP (PRPF19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRPF19 antibody included

经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, HeLa cells, human testis tissue, mouse liver tissue, PC-3 cells, rat kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRPF19 Ready-To-Use IHC Kit. PRPF19 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

15414-1-AP targets PRPF19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Immunogen PRPF19 fusion protein Ag7700 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Synonyms RING-type E3 ubiquitin transferase PRP19, PSO4, PRP19, Pre-mRNA-processing factor 19, hPso4
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC008719
Gene Symbol PRPF19
Gene ID (NCBI) 27339
RRIDAB_10637855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMS4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.

实验方案

Product Specific Protocols
WB protocol for PRPF19 antibody 15414-1-APDownload protocol
IHC protocol for PRPF19 antibody 15414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Expert Opin Ther Targets

Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice.

Authors - Pei Fan
View Article
humanWB

J Cell Biochem

Human RNF113A participates of pre-mRNA splicing in vitro.

Authors - Guilherme H Gatti da Silva
View Article
human,pigWB

J Virol

PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein

Authors - Xueying Zhai
View Article