PSEN1 Polyclonal antibody
PSEN1 Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | mouse brain tissue, HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy PSEN1/AD3 Ready-To-Use IHC Kit. PSEN1/AD3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
20434-1-AP targets PSEN1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF, IHC, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Immunogen | PSEN1 fusion protein Ag14135 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | presenilin 1 |
| Synonyms | AD3, FAD, presenilin 1, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182 |
| Calculated Molecular Weight | 467 aa, 53 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC011729 |
| Gene Symbol | PSEN1 |
| Gene ID (NCBI) | 5663 |
| RRID | AB_2878685 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a ~30-kDa N-terminal fragment (NTF) and a ~20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody can recognize all the isoforms except isoform 4 (21 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSEN1 antibody 20434-1-AP | Download protocol |
| IF protocol for PSEN1 antibody 20434-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Aging Neurosci LongShengZhi Capsule Attenuates Alzheimer-Like Pathology in APP/PS1 Double Transgenic Mice by Reducing Neuronal Oxidative Stress and Inflammation. |
