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  • KD/KO Validated

PTPN2 Polyclonal antibody

PTPN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11214-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

T-cell protein-tyrosine phosphatase, TC PTP, PTPT, PTPN 2, PTN2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inmouse thymus tissue, HeLa cells, HL-60 cells, NIH/3T3 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PTPN2 Ready-To-Use IHC Kit. PTPN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11214-1-AP targets PTPN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PTPN2 fusion protein Ag1709 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 2
Synonyms T-cell protein-tyrosine phosphatase, TC PTP, PTPT, PTPN 2, PTN2
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC016727
Gene Symbol PTPN2
Gene ID (NCBI) 5771
RRIDAB_2173235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17706
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN2 antibody 11214-1-APDownload protocol
IHC protocol for PTPN2 antibody 11214-1-APDownload protocol
IF protocol for PTPN2 antibody 11214-1-APDownload protocol
IP protocol for PTPN2 antibody 11214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Oncogene

Overexpression of TC-PTP in murine epidermis attenuates skin tumor formation.

Authors - Mihwa Kim

Biochim Biophys Acta Mol Basis Dis

PTPN2 dephosphorylates STAT3 to ameliorate anesthesia-induced cognitive decline in aged rats by altering the microglial phenotype and inhibiting inflammation

Authors - Xiaochun Zhao
human,mouseWB,IHC

Int Immunopharmacol

Hypoxia disrupts the nasal epithelial barrier by inhibiting PTPN2 in chronic rhinosinusitis with nasal polyps

Authors - Meiping Zhang
  • KD Validated
mouseIHC

Int Immunopharmacol

Oral squamous cell carcinoma-derived EVs promote tumor progression by regulating inflammatory cytokines and the IL-17A-induced signaling pathway

Authors - Ruowei Li
humanWB

Cell Death Dis

Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.

Authors - Liang Lu
  • KD Validated
mouseIHC

Sci Rep

Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development.

Authors - Hyunseung Lee
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