验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, COLO 320 cells, SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17937-1-AP targets PTPRR in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PTPRR fusion protein Ag12145 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, R |
| 别名 | Ch 1PTPase, EC PTP, ECPTP, NC PTPCOM1, PCPTP1, PTP SL, PTPBR7, PTPRQ, PTPRR, R PTP R |
| 计算分子量 | 657 aa, 74 kDa |
| 观测分子量 | 32 kDa, 47 kDa, 65 kDa |
| GenBank蛋白编号 | BC110900 |
| 基因名称 | PTPRR |
| Gene ID (NCBI) | 5801 |
| RRID | AB_10694430 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15256 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTPRR(protein tyrosine phosphatase, receptor type, R) is also named as PCPTP1, PTP-SL, PTPBR7 and belongs to the protein-tyrosine phosphatase family. It sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. It has four isoforms that they have molecular weights of 72, 60, 42 and 37 kDa, respectively, and differ in the length of their N-terminal part which result in distinct subcellular localizations. All PTPRR isoforms are found to be short-lived proteins, with half-lives of around 3-5 hours, and are constitutively phosphorylated on one or two of the previously identified protein kinase A and MAP kinase target sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPRR antibody 17937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Multi-cohort validation of Ascore: an anoikis-based prognostic signature for predicting disease progression and immunotherapy response in bladder cancer | ||
BMC Cancer Androgen-regulation of the protein tyrosine phosphatase PTPRR activates ERK1/2 signalling in prostate cancer cells. | ||
Front Mol Neurosci Downregulation of Protein Tyrosine Phosphatase Receptor Type R Accounts for the Progression of Hirschsprung Disease. |
