验证数据展示
经过测试的应用
Positive WB detected in | UV treated HEK-293T cells, LPS treated THP-1 cells, UV treated NIH/3T3 cells |
Positive IF/ICC detected in | LPS treated THP-1 cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28796-1-AP targets Phospho-p38 MAPK (Thr180/Tyr182) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase 14 |
Synonyms | p p38 MAPK, p p38 MAPK (Thr180/Tyr182), p38 alpha/MAPK14 (Thr180/Tyr182), p38 MAPK (Thr180/Tyr182), Phospho p38 MAPK |
Calculated Molecular Weight | 360 aa, 41 kDa |
Observed Molecular Weight | 38-42 kDa |
GenBank Accession Number | BC031574 |
Gene Symbol | p38 MAPK |
Gene ID (NCBI) | 1432 |
RRID | AB_2918205 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q16539 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-p38 MAPK (Thr180/Tyr182) antibody 28796-1-AP | Download protocol |
IF protocol for Phospho-p38 MAPK (Thr180/Tyr182) antibody 28796-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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