验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse brain tissue, rat brain tissue, HeLa cells, Neuro-2a cells, PC-12 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 2 publications below |
产品信息
10347-1-AP targets RAB4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | RAB4A fusion protein Ag0357 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RAB4A, member RAS oncogene family |
| Synonyms | Ras-related protein Rab-4A, Ras related protein Rab 4A, RAB4, HRES 1/RAB4, EC:3.6.5.2 |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 24-28 kDa |
| GenBank Accession Number | BC002438 |
| Gene Symbol | RAB4A |
| Gene ID (NCBI) | 5867 |
| RRID | AB_2177544 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20338 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human RAB genes share structural and biochemical properties with the Ras gene superfamily, and RAB4 (synonym: RAB4A) is a small GTPase belonging to this Ras superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. RAB4 may also function as regulator along the receptor recycling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB4A antibody 10347-1-AP | Download protocol |
| IHC protocol for RAB4A antibody 10347-1-AP | Download protocol |
| IF protocol for RAB4A antibody 10347-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
MBio Yersinia pestisTargets the Host Endosome Recycling Pathway during the Biogenesis of theYersinia-Containing Vacuole To Avoid Killing by Macrophages. | ||
Virology Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells. | ||
Pathog Dis Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages. | ||
FASEB J Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R. |
