验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, mouse colon tissue, SH-SY5Y cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
20228-1-AP targets RAB5A-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | RAB5A, member RAS oncogene family |
Synonyms | RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A |
Calculated Molecular Weight | 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | NM_004162 |
Gene Symbol | RAB5A |
Gene ID (NCBI) | 5868 |
RRID | AB_10694147 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20339 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.
实验方案
Product Specific Protocols | |
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WB protocol for RAB5A-Specific antibody 20228-1-AP | Download protocol |
IHC protocol for RAB5A-Specific antibody 20228-1-AP | Download protocol |
IP protocol for RAB5A-Specific antibody 20228-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |