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验证数据展示

  • WB analysis of human brain using 20228-1-AP

    WB analysis of human brain using 20228-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20228-1-AP (RAB5A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20228-1-AP

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 20228-1-AP

    IHC staining of human brain using 20228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20228-1-AP (RAB5A-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 20228-1-AP

    IP experiment of mouse brain using 20228-1-AP

    IP result of anti-RAB5A-Specific (IP:20228-1-AP, 4ug; Detection:20228-1-AP 1:600) with mouse brain tissue lysate 4000ug.

RAB5A-Specific Polyclonal antibody

RAB5A-Specific Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 20228-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse colon tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20228-1-AP targets RAB5A-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB5A, member RAS oncogene family
别名 RAB5, RAB5A, RAB5A-Specific, Ras related protein Rab 5A
计算分子量 24 kDa
观测分子量 24 kDa
GenBank蛋白编号NM_004162
基因名称 RAB5A
Gene ID (NCBI) 5868
RRIDAB_10694147
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20339
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB5A belongs to the small GTPase superfamily and Rab family. It is required for the fusion of plasma membranes and early endosomes. The antibody is specific to RAB5A. It has no cross reaction to RAB5B or RAB5C.

实验方案

Product Specific Protocols
WB protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IHC protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
IP protocol for RAB5A-Specific antibody 20228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Front Physiol

Overexpression of ameloblastin in secretory ameloblasts results in demarcated, hypomineralized opacities in enamel

Authors - Yong-Hee Patricia Chun
View Article