验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RAB9A Ready-To-Use IHC Kit. RAB9A primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11420-1-AP targets RAB9A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, hamster |
| Immunogen | RAB9A fusion protein Ag1969 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | RAB9A, member RAS oncogene family |
| Synonyms | RAB9, Ras-related protein Rab-9A, Ras related protein Rab 9A |
| Calculated Molecular Weight | 201 aa, 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC017265 |
| Gene Symbol | RAB9A |
| Gene ID (NCBI) | 9367 |
| RRID | AB_2175327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB9A antibody 11420-1-AP | Download protocol |
| IHC protocol for RAB9A antibody 11420-1-AP | Download protocol |
| IF protocol for RAB9A antibody 11420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci The Amyotrophic Lateral Sclerosis M114T PFN1 Mutation Deregulates Alternative Autophagy Pathways and Mitochondrial Homeostasis. | ||
Virology Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells. | ||
J Gen Virol Characterization of intracellular localization of PrP(Sc) in prion-infected cells using a mAb that recognizes the region consisting of aa 119-127 of mouse PrP. | ||
Technol Cancer Res Treat Knockdown of Rab9 Suppresses the Progression of Gastric Cancer Through Regulation of Akt Signaling Pathway.
| ||
J Virol Porcine Enteric Alphacoronavirus Entry through Multiple Pathways (Caveolae, Clathrin, and Macropinocytosis) Requires Rab GTPases for Endosomal Transport |
