验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, DU 145 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, PC-3 cells, A549 cells, K-562 cells, SH-SY5Y cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human lung cancer tissue, human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | LNCaP cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10638-1-AP targets REDD1 specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, chIP |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, pig, rabbit, squirrel, gerbil |
Immunogen | REDD1 specific fusion protein Ag0965 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DNA-damage-inducible transcript 4 |
Synonyms | DDIT4, REDD1, RTP801, REDD-1, HIF-1 responsive protein RTP801 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 32-35 kDa |
GenBank Accession Number | BC007714 |
Gene Symbol | REDD1/DDIT4 |
Gene ID (NCBI) | 54541 |
RRID | AB_2245711 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NX09 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927)
实验方案
Product Specific Protocols | |
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WB protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
IHC protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
IF protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
IP protocol for REDD1 specific antibody 10638-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1 | ||
Cancer Cell A UBE2O-AMPKα2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy. | ||
Nat Immunol The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity. |