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验证数据展示

  • WB analysis of MDA-MB-453s using 19671-1-AP

    WB analysis of MDA-MB-453s using 19671-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 19671-1-AP

    IHC staining of mouse kidney using 19671-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 19671-1-AP

    IHC staining of mouse kidney using 19671-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RHOG-Specific Polyclonal antibody

RHOG-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19671-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RHOG, ARHG, Rho-related GTP-binding protein RhoG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-453s cells, C6 cells, HeLa cells, MCF-7 cells, Neuro-2a cells, NIH/3T3 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19671-1-AP targets RHOG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras homolog gene family, member G (rho G)
Synonyms RHOG, ARHG, Rho-related GTP-binding protein RhoG
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberNM_001665
Gene Symbol RHOG
Gene ID (NCBI) 391
RRIDAB_2878593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP84095
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.

实验方案

Product Specific Protocols
WB protocol for RHOG-Specific antibody 19671-1-APDownload protocol
IHC protocol for RHOG-Specific antibody 19671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols