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验证数据展示

  • WB analysis of HeLa using 66028-1-Ig

    WB analysis of HeLa using 66028-1-Ig

    HeLa, Jurkat, L02 cells were subjected to SDS PAGE followed by western blot with 66028-1-Ig (RNH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 66028-1-Ig

    WB analysis of HeLa cells using 66028-1-Ig

    WB result of RNH1 antibody (66028-1-Ig, 1:4000) with si-control and si-RNH1 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

RNH1 Monoclonal antibody

RNH1 Monoclonal Antibody for WB, ELISA
Cat No. 66028-1-Ig
CloneNo. 4A1C4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH, RNH1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, L02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66028-1-Ig targets RNH1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RNH1 fusion protein Ag18093 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ribonuclease/angiogenin inhibitor 1
Synonyms Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH, RNH1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC000677
Gene Symbol RNH1
Gene ID (NCBI) 6050
RRIDAB_11043338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13489
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

实验方案

Product Specific Protocols
WB protocol for RNH1 antibody 66028-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols