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验证数据展示

  • WB analysis using 23599-1-AP

    WB analysis using 23599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23599-1-AP (RPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 23599-1-AP

    IHC staining of human breast cancer using 23599-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23599-1-AP (RPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 23599-1-AP

    IHC staining of human liver using 23599-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23599-1-AP (RPS25 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 23599-1-AP

    IF Staining of MCF-7 using 23599-1-AP

    Immunofluorescent analysis of MCF-7 cells using 23599-1-AP (RPS25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RPS25 Polyclonal antibody

RPS25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23599-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

40S ribosomal protein S25, ribosomal protein S25, RPS25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23599-1-AP targets RPS25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 RPS25 fusion protein Ag7890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S25
别名 40S ribosomal protein S25, ribosomal protein S25, RPS25
计算分子量 14 kDa
观测分子量 15-17 kDa
GenBank蛋白编号BC004986
基因名称 RPS25
Gene ID (NCBI) 6230
RRIDAB_11183757
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62851
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting.

实验方案

Product Specific Protocols
WB protocol for RPS25 antibody 23599-1-APDownload protocol
IHC protocol for RPS25 antibody 23599-1-APDownload protocol
IF protocol for RPS25 antibody 23599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Nano

Platelet-Derived Apoptotic Vesicles Promote Bone Regeneration via Golgi Phosphoprotein 2 (GOLPH2)-AKT Signaling Axis

Authors - Yuhe Jiang
View Article
humanWB

Mol Cell Proteomics

Identification and characterization of potential biomarkers by quantitative tissue proteomics of primary lung adenocarcinoma.

Authors - Chiung-Hung Hsu
View Article