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验证数据展示

  • IF Staining of HepG2 using CL488-66046

    IF Staining of HepG2 using CL488-66046

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66046 (RPS3 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RPS3 Monoclonal antibody

RPS3 Monoclonal Antibody for IF/ICC
Cat No. CL488-66046

产品说明书

CloneNo. 2G7H4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC

别名

40S ribosomal protein S3, FLJ26283, FLJ27450, ribosomal protein S3, Ribosome Marker, RPS3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPS3 Ready-To-Use IHC Kit. RPS3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66046-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66046 targets RPS3 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 RPS3 fusion protein Ag7515 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein S3
别名 40S ribosomal protein S3, FLJ26283, FLJ27450, ribosomal protein S3, Ribosome Marker, RPS3
计算分子量 27 kDa
观测分子量 33 kDa
GenBank蛋白编号BC003137
基因名称 RPS3
Gene ID (NCBI) 6188
RRIDAB_2883239
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23396
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPS3 antibody CL488-66046Download protocol
Standard Protocols
Click here to view our Standard Protocols