验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, MCF-7 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
14684-1-AP targets RXRB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | rat |
Immunogen | RXRB fusion protein Ag0263 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | retinoid X receptor, beta |
Synonyms | DAUDI6, H 2RIIBP, NR2B2, RCoR 1, Retinoid X receptor beta, retinoid X receptor, beta, RXRB |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 57 kDa, 70 kDa |
GenBank Accession Number | BC001167 |
Gene Symbol | RXRB |
Gene ID (NCBI) | 6257 |
RRID | AB_10858228 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P28702 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)
实验方案
Product Specific Protocols | |
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WB protocol for RXRB antibody 14684-1-AP | Download protocol |
IHC protocol for RXRB antibody 14684-1-AP | Download protocol |
IP protocol for RXRB antibody 14684-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |