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S100A5 Polyclonal antibody

S100A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17924-1-AP

Print datasheet

Synonyms

S100D, S100 calcium-binding protein A5, Protein S-100D, Protein S100-A5, Protein S100 A5

验证数据展示

  • WB analysis of HepG2 using 17924-1-AP

    WB analysis of HepG2 using 17924-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human meningioma using 17924-1-AP

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human meningioma using 17924-1-AP

    IHC staining of human meningioma using 17924-1-AP

    Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 17924-1-AP

    IF Staining of HepG2 using 17924-1-AP

    Immunofluorescent analysis of HepG2 cells using 17924-1-AP (S100A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human kidney tissue, human liver tissue, SH-SY5Y cells
Positive IHC detected inhuman meningioma tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17924-1-AP targets S100A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen S100A5 fusion protein Ag12342 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S100 calcium binding protein A5
Synonyms S100D, S100 calcium-binding protein A5, Protein S-100D, Protein S100-A5, Protein S100 A5
Calculated Molecular Weight 110 aa, 13 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC093955
Gene Symbol S100A5
Gene ID (NCBI) 6276
RRIDAB_2183794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for S100A5 antibody 17924-1-APDownload protocol
IHC protocol for S100A5 antibody 17924-1-APDownload protocol
IF protocol for S100A5 antibody 17924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Adv Sci (Weinh)

S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma

Authors - Huihuang Li
View Article
mouseIHC

Mol Cell Proteomics

The Membrane Proteome of Sensory Cilia to the Depth of Olfactory Receptors.

Authors - Katja Kuhlmann
View Article