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SEC24D Polyclonal antibody

SEC24D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13673-1-AP

Print datasheet

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755

验证数据展示

  • WB analysis using 13673-1-AP

    WB analysis using 13673-1-AP

    Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13673-1-AP (SEC24D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 13673-1-AP

    IHC staining of human pancreas using 13673-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 13673-1-AP

    IHC staining of human pancreas using 13673-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13673-1-AP (SEC24D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 13673-1-AP

    IF Staining of HeLa using 13673-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13673-1-AP (SEC24D antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13673-1-AP targets SEC24D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SEC24D fusion protein Ag4657 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC24 family, member D (S. cerevisiae)
Synonyms SEC24-related protein D, SEC24 related protein D, KIAA0755
Calculated Molecular Weight 1033 aa, 113 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC035761
Gene Symbol SEC24D
Gene ID (NCBI) 9871
RRIDAB_2877968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

实验方案

Product Specific Protocols
WB protocol for SEC24D antibody 13673-1-APDownload protocol
IHC protocol for SEC24D antibody 13673-1-APDownload protocol
IF protocol for SEC24D antibody 13673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang
View Article