验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, U2OS cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
产品信息
27684-1-AP targets SF3B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | SF3B1 fusion protein Ag26689 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | splicing factor 3b, subunit 1, 155kDa |
| 别名 | PRP10, PRPF10, SAP 155, SAP155, SF3B1, SF3b155, Splicing factor 3B subunit 1 |
| 计算分子量 | 146 kDa |
| 观测分子量 | 146-150 kDa |
| GenBank蛋白编号 | NM_012433 |
| 基因名称 | SF3B1 |
| Gene ID (NCBI) | 23451 |
| RRID | AB_2880946 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75533 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SF3B1 antibody 27684-1-AP | Download protocol |
| IHC protocol for SF3B1 antibody 27684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p. | ||
EMBO Rep A splicing factor switch controls hematopoietic lineage specification of pluripotent stem cells.
| ||
Sci Rep Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome | ||
Nat Commun Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12 | ||
EMBO J A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression | ||
Mol Cell Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles |
