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验证数据展示

  • IF Staining of mouse eye using 55059-1-AP

    IF Staining of mouse eye using 55059-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse eye using 55059-1-AP

    IF Staining of mouse eye using 55059-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 55059-1-AP

    WB analysis using 55059-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55059-1-AP (SHANK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293T using 55059-1-AP

    IP experiment of HEK-293T using 55059-1-AP

    IP result of anti-SHANK1 (IP:55059-1-AP, 4ug; Detection:55059-1-AP 1:400) with HEK-293T cells lysate 1155 ug.

SHANK1 Polyclonal antibody

SHANK1 Polyclonal Antibody for WB, IF-P, IP, ELISA
Cat No. 55059-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IP, ELISA

别名

SHANK1, SPANK 1, SSTR interacting protein, SSTRIP, synamon

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, Neuro-2a cells
Positive IP detected inHEK-293T cells
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55059-1-AP targets SHANK1 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 and multiple ankyrin repeat domains 1
别名 SHANK1, SPANK 1, SSTR interacting protein, SSTRIP, synamon
计算分子量 225 kDa
观测分子量 159-225 kDa
GenBank蛋白编号NM_016148
基因名称 SHANK1
Gene ID (NCBI) 50944
RRIDAB_10863130
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y566
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHANK1 belongs to the SHANK family. It seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. SHANK1 may play a role in the structural and functional organization of the dendritic spine and synaptic junction. The antibody is specific to SHANK1.

实验方案

Product Specific Protocols
WB protocol for SHANK1 antibody 55059-1-APDownload protocol
IF protocol for SHANK1 antibody 55059-1-APDownload protocol
IP protocol for SHANK1 antibody 55059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation.

Authors - Zeyu Zhang
View Article
ratWB

Neural Regen Res

Protective effects of Bushen Tiansui decoction on hippocampal synapses in a rat model of Alzheimer's disease.

Authors - Shan Hui
View Article