- Featured Product
- KD/KO Validated
NKCC2 Polyclonal antibody
NKCC2 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, 37°C incubated mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse Embryonic kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy NKCC2/SLC12A1 Ready-To-Use IHC Kit. NKCC2/SLC12A1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18970-1-AP targets NKCC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NKCC2 fusion protein Ag5325 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | solute carrier family 12 (sodium/potassium/chloride transporters), member 1 |
| Synonyms | BSC1, NKCC2, SLC12A1 |
| Calculated Molecular Weight | 121 kDa |
| Observed Molecular Weight | 135 kDa |
| GenBank Accession Number | BC040138 |
| Gene Symbol | SLC12A1/NKCC2 |
| Gene ID (NCBI) | 6557 |
| RRID | AB_2877130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13621 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NKCC2 antibody 18970-1-AP | Download protocol |
| IHC protocol for NKCC2 antibody 18970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk. | ||
Nat Protoc Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells | ||
Nat Commun Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids | ||
J Am Soc Nephrol A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids. | ||
J Am Soc Nephrol Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney. | ||
Mol Ther Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene. |
