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SLC25A17 Monoclonal antibody

SLC25A17 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D1D2

Cat no : 67635-1-Ig

Print datasheet

Synonyms

PMP34, SLC25A17

验证数据展示

  • WB analysis using 67635-1-Ig

    WB analysis using 67635-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human liver cancer using 67635-1-Ig

    IHC staining of human liver cancer using 67635-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, U2OS cells, A549 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67635-1-Ig targets SLC25A17 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen SLC25A17 fusion protein Ag24456 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein, 34kDa), member 17
Synonyms PMP34, SLC25A17
Calculated Molecular Weight 307 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC012998
Gene Symbol SLC25A17
Gene ID (NCBI) 10478
RRIDAB_2882836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43808
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A17 (solute carrier family 25 member 17), is a kind of peroxisomal transporter for multiple cofactors such as CoA, FAD, FMN and AMP. It May catalyze the transport of free CoA, FAD and NAD+ from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. SLC25A17 is the only member of the mitochondrial carrier family localized to peroxisomal. (PMID: 22185573, 32266253)

实验方案

Product Specific Protocols
WB protocol for SLC25A17 antibody 67635-1-IgDownload protocol
IHC protocol for SLC25A17 antibody 67635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols