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SLC7A4 Polyclonal antibody

SLC7A4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15748-1-AP

Print datasheet

Synonyms

VH, HCAT3, CAT-4, CAT4, CAT 4

验证数据展示

  • WB analysis of mouse brain using 15748-1-AP

    WB analysis of mouse brain using 15748-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15748-1-AP (SLC7A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15748-1-AP

    IHC staining of mouse brain using 15748-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15748-1-AP (SLC7A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15748-1-AP

    IF Staining of HeLa using 15748-1-AP

    Immunofluorescent analysis of Hela cells, using SLC7A4 antibody 15748-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse brain tissue, human liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15748-1-AP targets SLC7A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SLC7A4 fusion protein Ag8211 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 7 (cationic amino acid transporter, y+ system), member 4
Synonyms VH, HCAT3, CAT-4, CAT4, CAT 4
Calculated Molecular Weight 635 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008814
Gene Symbol SLC7A4
Gene ID (NCBI) 6545
RRIDAB_2190966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43246
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC7A4 antibody 15748-1-APDownload protocol
IHC protocol for SLC7A4 antibody 15748-1-APDownload protocol
IF protocol for SLC7A4 antibody 15748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The Identification of the Metabolism Subtypes of Skin Cutaneous Melanoma Associated With the Tumor Microenvironment and the Immunotherapy.

Authors - Ronghua Yang
View Article