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验证数据展示

  • WB analysis of HeLa using 51144-1-AP

    WB analysis of HeLa using 51144-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51144-1-AP (SMAD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 cells using 51144-1-AP

    WB analysis of HepG2 cells using 51144-1-AP

    WB results of SMAD4 antibody (51144-1-AP, 1:1000) with si-Control and si-SMAD4 transfected HepG2 cells.

  • IHC staining of human placenta using 51144-1-AP

    IHC staining of human placenta using 51144-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SMAD4 antibody included
  • IHC staining of human placenta using 51144-1-AP

    IHC staining of human placenta using 51144-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SMAD4 antibody included
  • Featured Product
  • KD/KO Validated

SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51144-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DPC4, hSMAD4, JIP, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman placenta tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

51144-1-AP targets SMAD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMAD family member 4
Synonyms DPC4, hSMAD4, JIP, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
RRIDAB_10918273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13485
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide (25-43aa) mapping within human SMAD4. It is specific to SMAD4.

实验方案

Product Specific Protocols
WB protocol for SMAD4 antibody 51144-1-APDownload protocol
IHC protocol for SMAD4 antibody 51144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Exp Ther Med

Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation.

Authors - Ruichen Wang
View Article
ratWB

Open Life Sci

NR4A1 inhibits the epithelial-mesenchymal transition of hepatic stellate cells: Involvement of TGF-β-Smad2/3/4-ZEB signaling.

Authors - Qian Huang
View Article
ratWB

Exp Ther Med

MicroRNA-122 inhibits epithelial-mesenchymal transition of hepatic stellate cells induced by the TGF-β1/Smad signaling pathway.

Authors - Bianqiao Cheng
View Article