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  • Featured Product
  • KD/KO Validated

SMPDL3B Polyclonal antibody

SMPDL3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16552-1-AP

Print datasheet

Synonyms

ASM like phosphodiesterase 3b, ASML3B, SMPDL3B

验证数据展示

  • WB analysis using 16552-1-AP

    WB analysis using 16552-1-AP

    COLO 320 cells lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 16552-1-AP

    WB analysis using 16552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16552-1-AP (SMPDL3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16552-1-AP

    IHC staining of human colon cancer using 16552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16552-1-AP

    IHC staining of human colon cancer using 16552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16552-1-AP

    IHC staining of human kidney using 16552-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 16552-1-AP

    IHC staining of human kidney using 16552-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16552-1-AP (SMPDL3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16552-1-AP targets SMPDL3B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Immunogen SMPDL3B fusion protein Ag9535 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sphingomyelin phosphodiesterase, acid-like 3B
Synonyms ASM like phosphodiesterase 3b, ASML3B, SMPDL3B
Calculated Molecular Weight373aa,42 kDa; 455aa,51 kDa
Observed Molecular Weight42 kDa, 50-65 kDa
GenBank Accession NumberBC014444
Gene Symbol SMPDL3B
Gene ID (NCBI) 27293
RRIDAB_2878276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92485
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMPDL3B is lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).

实验方案

Product Specific Protocols
WB protocol for SMPDL3B antibody 16552-1-APDownload protocol
IHC protocol for SMPDL3B antibody 16552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Mol Biosci

SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia

Authors - Huiqing Qu
  • KO Validated
View Article
ratWB,IHC

Basic Clin Pharmacol Toxicol

PMID: 29923306

Authors - Xin Li
View Article
mouseWB

JHEP Rep

Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease.

Authors - Taesik Gwag
View Article
human,pig,monkeyWB,IF

iScience

Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism

Authors - Huan-Huan Shen
  • KD Validated
View Article