SNF2L Polyclonal antibody
SNF2L Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | U-87 MG cells, U-251 cells, SH-SY5Y cells |
| Positive IHC detected in | mouse lung tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SMARCA1/SNF2L Ready-To-Use IHC Kit. SMARCA1/SNF2L primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29461-1-AP targets SNF2L in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SNF2L fusion protein Ag30657 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 |
| Synonyms | ATP dependent helicase SMARCA1, DKFZp686D1623, FLJ41547, ISWI, NURF140, SMARCA1, SNF2L, SNF2L1, SNF2LB, SWI, SWI2 |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 120-130 KDa |
| GenBank Accession Number | NM_003069 |
| Gene Symbol | SMARCA1/SNF2L |
| Gene ID (NCBI) | 6594 |
| RRID | AB_2918310 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28370 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNF2L antibody 29461-1-AP | Download protocol |
| IHC protocol for SNF2L antibody 29461-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
