验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells, human brain tissue, mouse skin tissue, mouse brain tissue, mouse cerebellum tissue, RAW 264.7 cells, SK-N-SH cells, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10275-1-AP targets SNX17 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SNX17 fusion protein Ag0355 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorting nexin 17 |
| 别名 | Sorting nexin-17, KIAA0064 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC002524 |
| 基因名称 | SNX17 |
| Gene ID (NCBI) | 9784 |
| RRID | AB_2192394 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15036 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled-coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin and may function in the intracellular trafficking of P-selectin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX17 antibody 10275-1-AP | Download protocol |
| IHC protocol for SNX17 antibody 10275-1-AP | Download protocol |
| IP protocol for SNX17 antibody 10275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.
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Nat Cell Biol Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling. | ||
Nat Cell Biol Recycling of autophagosomal components from autolysosomes by the recycler complex. | ||
Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. | ||
Proc Natl Acad Sci U S A LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.
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