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- KD/KO Validated
SREBF1 Monoclonal antibody
SREBF1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B6G5
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, Jurkat cells, HeLa cells, MCF-7 cells, L02 cells, HT-29 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66875-1-Ig targets SREBF1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, IHC, WB, ELISA Application Description |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | Human |
Cited Reactivity | human, bovine |
Immunogen | SREBF1 fusion protein Ag5484 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | sterol regulatory element binding transcription factor 1 |
Synonyms | bHLHd1, SREBF1, SREBP 1, SREBP 1c, SREBP1 |
Calculated Molecular Weight | 1177 aa, 125 kDa |
Observed Molecular Weight | 125 kDa |
GenBank Accession Number | BC063281 |
Gene Symbol | SREBF1 |
Gene ID (NCBI) | 6720 |
RRID | AB_2882209 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P36956 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SREBF1 antibody 66875-1-Ig | Download protocol |
IHC protocol for SREBF1 antibody 66875-1-Ig | Download protocol |
IF protocol for SREBF1 antibody 66875-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Nat Commun Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease | ||
Phytomedicine Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis | ||
Phytomedicine Asperosaponin VI protects alcohol-induced hepatic steatosis and injury via regulating lipid metabolism and ER stress | ||
J Hazard Mater In vitro effects of Triclocarban on adipogenesis in murine preadipocyte and human hepatocyte. |