SUGT1 Polyclonal antibody

SUGT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11675-1-AP

Print datasheet

Synonyms

Sgt1, Protein SGT1 homolog, Protein 40-6-3, Protein 40 6 3



经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SUGT1 Ready-To-Use IHC Kit. SUGT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11675-1-AP targets SUGT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SUGT1 fusion protein Ag2275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
Synonyms Sgt1, Protein SGT1 homolog, Protein 40-6-3, Protein 40 6 3
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38 kDa and 41 kDa
GenBank Accession NumberBC000911
Gene Symbol SUGT1
Gene ID (NCBI) 10910
RRIDAB_2197436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Z0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SUGT1 antibody 11675-1-APDownload protocol
IHC protocol for SUGT1 antibody 11675-1-APDownload protocol
IF protocol for SUGT1 antibody 11675-1-APDownload protocol
IP protocol for SUGT1 antibody 11675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

HSF1 Protects Sepsis-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome Activation.

Authors - Xueyan Shi
humanIHC

Oncogenesis

The oncogenic role of the cochaperone Sgt1.

Authors - H Ogi
humanWB

PLoS One

Calcyclin Binding Protein/Siah-1 Interacting Protein Is a Hsp90 Binding Chaperone.

Authors - Agnieszka Góral
humanIHC

Int J Oncol

Overexpression of SUGT1 in human colorectal cancer and its clinicopathological significance.

Authors - Iwatsuki Masaaki M
humanWB

JCI Insight

Echinatin effectively protects against NLRP3 inflammasome-driven diseases by targeting HSP90.

Authors - Guang Xu