验证数据展示
经过测试的应用
| Positive WB detected in | NK-92 cells, A549 cells, mouse thymus tissue, RAW 264.7 cells |
| Positive IP detected in | mouse thymus tissue |
| Positive FC (Intra) detected in | NK92 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13700-1-AP targets TBX21/T-bet in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | TBX21/T-bet fusion protein Ag4618 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | T-box 21 |
| Synonyms | TBX21, TBX21,T-bet, TBX21,T bet, TBX 21, T-box transcription factor TBX21 |
| Calculated Molecular Weight | 535 aa, 58 kDa |
| Observed Molecular Weight | 60-62 kDa |
| GenBank Accession Number | BC039739 |
| Gene Symbol | TBX21/T-bet |
| Gene ID (NCBI) | 30009 |
| ENSEMBL Gene ID | ENSG00000073861 |
| RRID | AB_10666436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| IP protocol for TBX21/T-bet antibody 13700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.
| ||
Int Immunopharmacol Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway | ||
Int J Cardiol Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome. | ||
Mol Nutr Food Res A High MCT-Based Ketogenic Diet Suppresses Th1 and Th17 Responses to Ameliorate Experimental Autoimmune Encephalomyelitis in Mice by Inhibiting GSDMD and JAK2-STAT3/4 Pathways | ||
Biomed Pharmacother Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity. | ||
Front Pharmacol Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy. |
