验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse brain tissue, JAR cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14519-1-AP targets TCF7L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Immunogen | TCF7L1 fusion protein Ag5983 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transcription factor 7-like 1 (T-cell specific, HMG-box) |
| Synonyms | Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3 |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 63-70 kDa |
| GenBank Accession Number | BC058894 |
| Gene Symbol | TCF7L1 |
| Gene ID (NCBI) | 83439 |
| RRID | AB_2287033 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCS4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF7L1 antibody 14519-1-AP | Download protocol |
| IHC protocol for TCF7L1 antibody 14519-1-AP | Download protocol |
| IF protocol for TCF7L1 antibody 14519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal | ||
Nat Commun Wnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling. | ||
Stem Cell Reports Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta. | ||
Oncogenesis TCF7L1 regulates cytokine response and neuroendocrine differentiation of prostate cancer. | ||
iScience Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity | ||
Endocrinology Wnt-dependent T-cell factor-4 controls human extravillous trophoblast motility. |
