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TEC Polyclonal antibody

TEC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18085-1-AP

Print datasheet

Synonyms

PSCTK4, TEC, tec protein tyrosine kinase, Tyrosine protein kinase Tec

验证数据展示

  • WB analysis using 18085-1-AP

    WB analysis using 18085-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18085-1-AP (TEC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 18085-1-AP

    IHC staining of human lung cancer using 18085-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18085-1-AP (TEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, mouse kidney tissue, Jurkat cells, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 18085-1-AP is TEC Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TEC fusion protein Ag12804 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tec protein tyrosine kinase
Synonyms PSCTK4, TEC, tec protein tyrosine kinase, Tyrosine protein kinase Tec
Calculated Molecular Weight 631 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC101711
Gene Symbol TEC
Gene ID (NCBI) 7006
RRIDAB_2878494
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TEC antibody 18085-1-APDownload protocol
IHC protocol for TEC antibody 18085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols