验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse testis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TNKS Ready-To-Use IHC Kit. TNKS primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18030-1-AP targets TNKS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TNKS fusion protein Ag12597 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase |
| 别名 | PARP5A, PARP 5a, EC:2.4.2.30, EC:2.4.2.-, ARTD5 |
| 计算分子量 | 1327 aa, 142 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC098394 |
| 基因名称 | TNKS |
| Gene ID (NCBI) | 8658 |
| RRID | AB_2303564 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95271 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNKS antibody 18030-1-AP | Download protocol |
| IHC protocol for TNKS antibody 18030-1-AP | Download protocol |
| IF protocol for TNKS antibody 18030-1-AP | Download protocol |
| IP protocol for TNKS antibody 18030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation | ||
Oncol Lett Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer. | ||
Cell Death Dis circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.
| ||
Chem Biol Interact Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer |
