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TNRC4 Polyclonal antibody

TNRC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25353-1-AP

Print datasheet

Synonyms

Bruno like protein 1, BRUNOL1, CAG repeat protein 4, CAGH4, CELF 3, CELF3, ERDA4, ETR 1, RNA binding protein BRUNOL 1, TNRC4

验证数据展示

  • WB analysis of mouse lung using 25353-1-AP

    WB analysis of mouse lung using 25353-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 25353-1-AP

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CELF3 antibody included
  • IHC staining of human testis using 25353-1-AP

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CELF3 antibody included

经过测试的应用

Positive WB detected inmouse lung tissue, mouse spleen tissue
Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CELF3 Ready-To-Use IHC Kit. CELF3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25353-1-AP is TNRC4 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TNRC4 fusion protein Ag18601 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trinucleotide repeat containing 4
Synonyms Bruno like protein 1, BRUNOL1, CAG repeat protein 4, CAGH4, CELF 3, CELF3, ERDA4, ETR 1, RNA binding protein BRUNOL 1, TNRC4
Calculated Molecular Weight 464 aa, 51 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC052491
Gene Symbol TNRC4
Gene ID (NCBI) 11189
RRIDAB_2880041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SZQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNRC4, also named as BRUNOL1, CAGH4, ERDA4, or TNRC4, is a 465 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the CELF/BRUNOL family. TNRC4 localizes in the nucleus and is expressed in brain. TNRC4 as a RNA-binding protein is involved in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing.

实验方案

Product Specific Protocols
WB protocol for TNRC4 antibody 25353-1-APDownload protocol
IHC protocol for TNRC4 antibody 25353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols