验证数据展示
经过测试的应用
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| IHC | See 6 publications below |
产品信息
20987-1-AP targets TRPV4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transient receptor potential cation channel, subfamily V, member 4 |
| 别名 | Osm 9 like TRP channel 4, OTRPC4, TRP12, TRPV4, VR OAC, VRL 2, VRL2, VROAC |
| 计算分子量 | 98 kDa |
| GenBank蛋白编号 | NM_021625 |
| 基因名称 | TRPV4 |
| Gene ID (NCBI) | 59341 |
| RRID | AB_10926386 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HBA0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRPV4 antibody 20987-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.
| ||
Cell Death Dis Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma | ||
Environ Pollut Exposure to both formaldehyde and high relative humidity exacerbates allergic asthma by activating the TRPV4-p38 MAPK pathway in Balb/c mice. | ||
Neuropharmacology TRPV4 inhibitor HC067047 produces antidepressant-like effect in LPS-induced depression mouse model. | ||
Cell Mol Bioeng The mechanosensitive ion channel TRPV4 is a regulator of lung development and pulmonary vasculature stabilization. |