验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, HEK-293 cells, human brain tissue, human heart tissue, human liver tissue, human spleen tissue, human serum |
| Positive IHC detected in | mouse colon tissue, mouse heart tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11891-1-AP targets Prealbumin/transthyretin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Prealbumin/transthyretin fusion protein Ag2499 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transthyretin |
| 别名 | Prealbumin, transthyretin, TTR, TBPA, PALB |
| 计算分子量 | 147 aa, 16 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC020791 |
| 基因名称 | TTR |
| Gene ID (NCBI) | 7276 |
| RRID | AB_2212083 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02766 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Prealbumin/transthyretin antibody 11891-1-AP | Download protocol |
| IHC protocol for Prealbumin/transthyretin antibody 11891-1-AP | Download protocol |
| IF protocol for Prealbumin/transthyretin antibody 11891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS. | ||
Theranostics Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4. | ||
Int J Mol Sci Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest. |
