验证数据展示

WB analysis of A375 using 12869-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of mouse kidney using 12869-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 12869-1-AP
Immunofluorescent analysis of frozen mouse kidney tissue fixed with 4% PFA for 24 hours, embedded in OCT, permeabilized with 0.5% Triton X-100 PBS and subjected to antigen retrieval in sodium citrate buffer (pH 6.0) using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of A375 using 12869-1-AP
IP result of anti-UGCG (IP:12869-1-AP, 4ug; Detection:12869-1-AP 1:500) with A375 cells lysate 3200ug.
IHC staining of human lung cancer using 12869-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12869-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 12869-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO验证

UGCG Polyclonal antibody

UGCG Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Cat No. 12869-1-AP

产品说明书

引用文献(12)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, IP, ELISA and More (1)

别名

Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

规格:

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概述

经过测试的应用
产品信息
发表文章 (12)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A375 cells, C6 cells, mouse brain tissue, U-251 cells
Positive IP detected in	A375 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue, mouse brain tissue
Positive IF-Fro detected in	mouse kidney tissue

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 3 publications below
IF	See 5 publications below

产品信息

12869-1-AP targets UGCG in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF-P, IF-Fro, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	UGCG fusion protein Ag3530 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	UDP-glucose ceramide glucosyltransferase
别名	Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80
计算分子量	394 aa, 45 kDa
观测分子量	50-55 kDa
GenBank蛋白编号	BC038711
基因名称	UGCG
Gene ID (NCBI)	7357
RRID	AB_10915469
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q16739
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.

实验方案

Product Specific Protocols
WB protocol for UGCG antibody 12869-1-AP	Download protocol
IHC protocol for UGCG antibody 12869-1-AP	Download protocol
IF protocol for UGCG antibody 12869-1-AP	Download protocol
IP protocol for UGCG antibody 12869-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nat Commun Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids Authors - Junchen Liu View Article
human,mouse	WB	Cancer Res Aneuploid cell survival relies upon sphingolipid homeostasis. Authors - Yun-Chi Tang KD Validated View Article
mouse	WB,IF	Cell Mol Biol Lett UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway Authors - Shengyu Cui View Article
human, mouse	WB,IHC	Hum Mol Genet Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase. Authors - Alexandre Henriques View Article
human	WB,IHC	Aging (Albany NY) Identification of long-term survival-associated gene in breast cancer. Authors - Shipeng Ning View Article
human	IF	iScience Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation. Authors - Zhiqiang Li View Article

验证数据展示

WB analysis of A375 using 12869-1-AP

IF Staining of mouse kidney using 12869-1-AP

IF Staining of mouse kidney using 12869-1-AP

IP experiment of A375 using 12869-1-AP

IHC staining of human lung cancer using 12869-1-AP

IHC staining of human lung cancer using 12869-1-AP

IF Staining of mouse brain using 12869-1-AP

UGCG Polyclonal antibody

UGCG Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids

Aneuploid cell survival relies upon sphingolipid homeostasis.

UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway

Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.

Identification of long-term survival-associated gene in breast cancer.

Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.