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  • KD/KO Validated

ULK1 Polyclonal antibody

ULK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20986-1-AP

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Synonyms

ATG1, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1



经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, HepG2 cells, HEK-293T cells, rat skeletal muscle tissue, HeLa cells
Positive IHC detected inhuman skeletal muscle tissue, human liver cancer tissue, human liver tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20986-1-AP targets ULK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-51-like kinase 1 (C. elegans)
Synonyms ATG1, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1
Calculated Molecular Weight 113 kDa
Observed Molecular Weight113-140 kDa
GenBank Accession NumberNM_003565
Gene Symbol ULK1
Gene ID (NCBI) 8408
RRIDAB_2878783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75385
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

实验方案

Product Specific Protocols
WB protocol for ULK1 antibody 20986-1-APDownload protocol
IHC protocol for ULK1 antibody 20986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Cell

Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.

Authors - Hua Su
humanWB

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
  • KO Validated
humanWB

Autophagy

Topology-dependent, bifurcated mitochondrial quality control under starvation.

Authors - Yanshuang Zhou
humanIF

Dev Cell

Glucose starvation induces autophagy via ULK1-mediated activation of PIKfyve in an AMPK-dependent manner.

Authors - Cansu Karabiyik
humanWB

J Hazard Mater

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.

Authors - Feng Xiaoli
mouseWB

Cell Rep

Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis

Authors - Katyayanee Neopane