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验证数据展示

  • WB analysis using 15547-1-AP

    WB analysis using 15547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 15547-1-AP

    IF Staining of HEK-293 using 15547-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UROD antibody (15547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human kidney using 15547-1-AP

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 15547-1-AP

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of K-562 using 15547-1-AP

    IP experiment of K-562 using 15547-1-AP

    IP result of anti-UROD (IP:15547-1-AP, 4ug; Detection:15547-1-AP 1:500) with K-562 cells lysate 4400 ug.

UROD Polyclonal antibody

UROD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15547-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

URO-D, EC:4.1.1.37

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, rat kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15547-1-AP targets UROD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 UROD fusion protein Ag7905 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uroporphyrinogen decarboxylase
别名 URO-D, EC:4.1.1.37
计算分子量 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC001778
基因名称 UROD
Gene ID (NCBI) 7389
RRIDAB_2213813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06132
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UROD antibody 15547-1-APDownload protocol
IHC protocol for UROD antibody 15547-1-APDownload protocol
IF protocol for UROD antibody 15547-1-APDownload protocol
IP protocol for UROD antibody 15547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
View Article
mouseWB

Cell Mol Gastroenterol Hepatol

The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria

Authors - Ning Kuo
View Article