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VCAM-1 Polyclonal antibody

VCAM-1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11444-1-AP

Print datasheet

Synonyms

CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1



经过测试的应用

Positive WB detected inTNF alpha treated HUVEC cells
Positive IHC detected inhuman tonsillitis tissue, human kidney tissue, human spleen tissue, human placenta tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11444-1-AP targets VCAM-1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited Applications FC, IF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen VCAM-1 fusion protein Ag1993 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name vascular cell adhesion molecule 1
Synonyms CD106, INCAM 100, V CAM 1, VCAM 1, VCAM1, VCAM-1
Calculated Molecular Weight 739 aa, 81 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC017276
Gene Symbol VCAM1
Gene ID (NCBI) 7412
RRIDAB_2214232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19320
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).

实验方案

Product Specific Protocols
WB protocol for VCAM-1 antibody 11444-1-APDownload protocol
IHC protocol for VCAM-1 antibody 11444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Cardiovasc Res

HDAC4 regulates vascular inflammation via activation of autophagy.

Authors - Di Yang
humanIF

EBioMedicine

Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion.

Authors - Peng Zhou
IHC

Aging Dis

Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.

Authors - Danyang Li
human

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
IF

Br J Pharmacol

Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation

Authors - Hang Su

Theranostics

Ligand Activation of PPARγ by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1α.

Authors - Feng Zhang