VHL Polyclonal antibody

VHL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24756-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (3)

HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, Raji cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VHL Ready-To-Use IHC Kit. VHL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24756-1-AP targets VHL in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen VHL fusion protein Ag21447 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name von Hippel-Lindau tumor suppressor
Synonyms HRCA1, Protein G7, pVHL, RCA1, VHL, VHL1
Calculated Molecular Weight 172 aa, 20 kDa
Observed Molecular Weight 18-24 kDa
GenBank Accession NumberBC058831
Gene Symbol VHL
Gene ID (NCBI) 7428
RRIDAB_2879705
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40337
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228). VHL has 3 isoforms with the molecular mass of 24, 20 and 18 kDa.

实验方案

Product Specific Protocols
WB protocol for VHL antibody 24756-1-APDownload protocol
IHC protocol for VHL antibody 24756-1-APDownload protocol
IF protocol for VHL antibody 24756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Hypoxia-Induced LncRNA-MIR210HG Promotes Cancer Progression By Inhibiting HIF-1α Degradation in Ovarian Cancer.

Authors - Ping Liu
humanWB

Microvasc Res

A positive feedback loop between miR-574-3p and HIF-1α in promoting angiogenesis under hypoxia

Authors - Renwen Zhang
CoIP

Cell Death Discov

Septin4 promotes cardiomyocytes apoptosis by enhancing the VHL-mediated degradation of HIF-1α.

Authors - Shaojun Wu
humanWB,CoIP

Pathol Oncol Res

MDM2 promotes cancer cell survival through regulating the expression of HIF-1α and pVHL in retinoblastoma

Authors - Shouhua Zhang
humanWB

EMBO J

A mitochondrial EglN1-AMPKα axis drives breast cancer progression by enhancing metabolic adaptation to hypoxic stress

Authors - Weiwei Jiang
humanIHC,WB

Cancer Res

Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression

Authors - Lijie Zhou
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