验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human heart tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
23108-1-AP targets XRN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | XRN1 fusion protein Ag19398 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | 5'-3' exoribonuclease 1 |
| Synonyms | 5 3 exoribonuclease 1, 5' 3' exoribonuclease 1, SEP1, XRN1 |
| Calculated Molecular Weight | 1706 aa, 194 kDa |
| Observed Molecular Weight | 175 kDa |
| GenBank Accession Number | BC078146 |
| Gene Symbol | XRN1 |
| Gene ID (NCBI) | 54464 |
| RRID | AB_2879211 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Exoribonuclease I (XRN1), also known as Sep1 or Rar5, is a 1,694-amino acid protein that functions as the major cytoplasmic 5 prime to 3 prime exoribonuclease and plays an important role in mRNA turnover. XRN1 may also function in the microtubular cytoskeleton as well as in DNA recombination and replication. XRN1 induces the expression of stress granules (SGs), cytoplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress, and GW bodies/processing bodies (PBs), distinct cytoplasmic sites of mRNA degradation. There are several isoforms of XRN1 that are produced as a result of alternative splicing events.Loss of XRN1 markedly affects cell growth rates.This antibody is specific to the XRN1 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XRN1 antibody 23108-1-AP | Download protocol |
| IHC protocol for XRN1 antibody 23108-1-AP | Download protocol |
| IF protocol for XRN1 antibody 23108-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer | ||
Nucleic Acids Res LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential
|
