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验证数据展示

  • IP experiment of human plasma using 18052-1-AP

    IP experiment of human plasma using 18052-1-AP

    IP result of anti-fetuin-B (IP:18052-1-AP, 4ug; Detection:18052-1-AP 1:2000) with human plasma lysate 4000 ug.

  • WB analysis of human plasma using 18052-1-AP

    WB analysis of human plasma using 18052-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18052-1-AP

    IHC staining of human liver cancer using 18052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

fetuin-B Polyclonal antibody

fetuin-B Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18052-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

FETUB, 16G2, Gugu, Fetuin-like protein IRL685, FetuinB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman blood tissue, human plasma
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18052-1-AP targets fetuin-B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 fetuin-B fusion protein Ag12562 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fetuin B
别名 FETUB, 16G2, Gugu, Fetuin-like protein IRL685, FetuinB
计算分子量 382 aa, 42 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC074734
基因名称 Fetuin B
Gene ID (NCBI) 26998
RRIDAB_2103181
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGM5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

实验方案

Product Specific Protocols
WB protocol for fetuin-B antibody 18052-1-APDownload protocol
IHC protocol for fetuin-B antibody 18052-1-APDownload protocol
IP protocol for fetuin-B antibody 18052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols