验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, Jurkat cells, MCF-7 cells, K-562 cells, Hek-293 cells, HeLa cells, Raji cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human colon tissue, human liver cancer tissue, human stomach tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, TNF alpha treated HT-1080 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10745-1-AP targets NF-κB p65 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP, chIP, ELISA |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, rabbit, monkey, chicken, hamster, sheep, goat, fish |
免疫原 | NF-κB p65 fusion protein Ag1199 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
别名 | NF κB, p65, p65; RELA, RELA, NF Kappa B |
计算分子量 | 65 kDa |
观测分子量 | 65 kDa |
GenBank蛋白编号 | BC011603 |
基因名称 | NF-κB p65 |
Gene ID (NCBI) | 5970 |
RRID | AB_2178878 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q04206 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.
实验方案
Product Specific Protocols | |
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WB protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
IHC protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
IF protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
IP protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
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