验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, A2780 cells |
| Positive IP detected in | SKOV-3 cells |
| Positive IHC detected in | human ovary cancer tissue, human kidney tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SKOV-3 cells |
Planning an IHC experiment? We recommend our IHCeasy PAX8 Ready-To-Use IHC Kit. PAX8 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 3 publications below |
| IF | See 2 publications below |
产品信息
21383-1-AP targets Pan-PAX in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | Pan-PAX fusion protein Ag16018 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | paired box 8 |
| 别名 | PAX-8, PAX8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8 |
| 计算分子量 | 450 aa, 48 kDa |
| 观测分子量 | 36-48 kDa |
| GenBank蛋白编号 | BC001060 |
| 基因名称 | PAX8 |
| Gene ID (NCBI) | 7849 |
| RRID | AB_10733094 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q06710 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Pan-PAX antibody 21383-1-AP | Download protocol |
| IHC protocol for Pan-PAX antibody 21383-1-AP | Download protocol |
| IF protocol for Pan-PAX antibody 21383-1-AP | Download protocol |
| IP protocol for Pan-PAX antibody 21383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy. | ||
Sci Signal Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. | ||
Am J Surg Pathol PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract. | ||
