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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TGF beta 3 protein

Activity

0.01 - 0.2 ng/mL

Specific Activity

minimally 1.00 x 107 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat No : HZ-1090

Print datasheet

Synonyms

ARVD, FLJ16571, TGF beta 3, TGF beta3, TGFB3

Validation Data Gallery

  • Purity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human TGF beta 3 (HZ-1090) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.14-0.75 ng/mL

    Recombinant human TGF beta 3 (HZ-1090) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.14-0.75 ng/mL

Technical Specifications

GeneID 7043
Species Human
Expression HEK293
Activity 0.01 - 0.2 ng/mL
Specific Activityminimally 1.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP17125
Molecular Mass 13 kDa reduced, 22 kDa non-reduced, homodimer, non-glycosylated
Formulation 50 mM NaOAc pH 3.7 + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.1mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

The transforming growth factor beta-3 (TGFβ-3) protein is found throughout the body and is required for development before birth and throughout life. TGFβ-3 binds to the receptor proteins on the surface of cells. This binding triggers the transmission of signals within the cell. As part of the TGF-β a signalling pathway, the TGFβ-3 protein helps to control the growth and cell proliferation. The TGFβ-3 protein is especially abundant in skeletal muscles and plays a key role in their development. The protein is also involved in blood vessels formation, regulation of bone growth, and wound healing (PMID: 25835445; 23824657)

Synonyms

ARVD, FLJ16571, TGF beta 3, TGF beta3, TGFB3

Publications

SpeciesTitle
rat

Biology (Basel)

Myogenic Differentiation and Immunomodulatory Properties of Rat Adipose-Derived Mesenchymal Stem/Stromal Cells

Authors - Sai Koung Ngeun
View Article
human

Biochem J

Soluble CD109 binds TGF-β and antagonizes TGF-β signalling and responses.

Authors - CarterLi
View Article
human

Front Bioeng Biotechnol

Mechano-Hypoxia Conditioning of Engineered Human Meniscus.

Authors - Alexander R A Szojka
View Article
human

Biol Reprod

Constitutive activation of transforming growth factor Beta receptor 1 in the mouse uterus impairs uterine morphology and function.

Authors - YangGao
View Article
human

PLoS One

Oxygen tension is a determinant of the matrix-forming phenotype of cultured human meniscal fibrochondrocytes.

Authors - Adetola BAdesida
View Article
mouse

PLoS One

YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.

Authors - RainerSchmid
View Article