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Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26195-1-AP

Print datasheet

Synonyms

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1

验证数据展示

  • IF Staining of C2C12 using 26195-1-AP

    IF Staining of C2C12 using 26195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of C2C12 using 26195-1-AP

    IF Staining of C2C12 using 26195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of MDCK using 26195-1-AP

    IF Staining of MDCK using 26195-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse brain using 26195-1-AP

    WB analysis of mouse brain using 26195-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 26195-1-AP

    IHC staining of human skin cancer using 26195-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 26195-1-AP (Gamma Tubulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells, MDCK cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26195-1-AP targets Gamma Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, canine
Immunogen Gamma Tubulin fusion protein Ag23575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, gamma 1
Synonyms TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC000619
Gene Symbol Gamma Tubulin
Gene ID (NCBI) 7283
RRIDAB_2880420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

实验方案

Product Specific Protocols
WB protocol for Gamma Tubulin antibody 26195-1-APDownload protocol
IHC protocol for Gamma Tubulin antibody 26195-1-APDownload protocol
IF protocol for Gamma Tubulin antibody 26195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols