验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HeLa cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | C2C12 cells, MDCK cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26195-1-AP targets Gamma Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, canine |
Immunogen | Gamma Tubulin fusion protein Ag23575 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tubulin, gamma 1 |
Synonyms | TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1 |
Calculated Molecular Weight | 51 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC000619 |
Gene Symbol | Gamma Tubulin |
Gene ID (NCBI) | 7283 |
RRID | AB_2880420 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P23258 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.
实验方案
Product Specific Protocols | |
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WB protocol for Gamma Tubulin antibody 26195-1-AP | Download protocol |
IHC protocol for Gamma Tubulin antibody 26195-1-AP | Download protocol |
IF protocol for Gamma Tubulin antibody 26195-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |