ubiquitin Monoclonal antibody
ubiquitin Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D7B2
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
60310-1-Ig targets ubiquitin in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | ubiquitin fusion protein Ag0260 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ubiquitin B |
Synonyms | ubiquitin |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC000379 |
Gene Symbol | ubiquitin |
Gene ID (NCBI) | 7314 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P0CG47 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ubiquitin antibody 60310-1-Ig | Download protocol |
IHC protocol for ubiquitin antibody 60310-1-Ig | Download protocol |
IF protocol for ubiquitin antibody 60310-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Lett Exosome-mediated transfer of SNHG7 enhances docetaxel resistance in lung adenocarcinoma | ||
Cell Death Differ Parkin maintains mitochondrial levels of the protective Parkinson's disease-related enzyme 17-β hydroxysteroid dehydrogenase type 10. | ||
Autophagy Sensitive ELISA-based detection method for the mitophagy marker p-S65-Ub in human cells, autopsy brain, and blood samples |