验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10187-2-AP targets RAB6A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | RAB6A fusion protein Ag0240 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB6A, member RAS oncogene family |
| 别名 | Ras-related protein Rab-6A, Ras related protein Rab 6A, Rab-6, RAB6, Rab 6 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC003617 |
| 基因名称 | RAB6A |
| Gene ID (NCBI) | 5870 |
| RRID | AB_2175463 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20340 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Members of the Rab subfamily of small GTPases play an important role in the regulation of intracellular transport routes. Rab6, localized to the Golgi apparatus, has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). The expression of Rab6 is ubiquitous.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB6A antibody 10187-2-AP | Download protocol |
| IF protocol for RAB6A antibody 10187-2-AP | Download protocol |
| IP protocol for RAB6A antibody 10187-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. | ||
J Neuroimmunol Autoantibody against the Rab6A/Rab6B in primary autoimmune cerebellar ataxia associated with Sjogren's syndrome: A case report. | ||
Neurosci Lett Absence of motor impairments or pathological changes in TMEM230 knockout rats | ||
Development A crucial role for Arf6 in the response of commissural axons to Slit.
| ||
Nat Commun SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. |
