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验证数据展示

  • IHC staining of mouse testis using 10496-1-AP

    IHC staining of mouse testis using 10496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10496-1-AP (HOXD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 10496-1-AP

    IF Staining of U2OS using 10496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HOXD3 antibody (10496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

HOXD3 Polyclonal antibody

HOXD3 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 10496-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Hox 4.1, Homeobox protein Hox-D3, Homeobox protein Hox-4A, Homeobox protein Hox D3, Homeobox protein Hox 4A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10496-1-AP targets HOXD3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen HOXD3 fusion protein Ag0768 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homeobox D3
Synonyms Hox 4.1, Homeobox protein Hox-D3, Homeobox protein Hox-4A, Homeobox protein Hox D3, Homeobox protein Hox 4A
Calculated Molecular Weight 46 kDa
GenBank Accession NumberBC005124
Gene Symbol HOXD3
Gene ID (NCBI) 3232
RRIDAB_2233224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homeobox D3 (HOXD3), a member of the Homeobox (Hox) family of master transcription factors, is a homeobox transcription factor that promotes angiogenesis and collagen synthesis. HOXD3 is expressed during normal embryogenesis, skin development, and fetal wound healing (PMID: 14633614). The overexpression of HOXD3 plays a critical role in breast cancer stemness and drug resistance via integrin β3-mediated Wnt/β-catenin signaling (PMID: 29698974).

实验方案

Product Specific Protocols
IHC protocol for HOXD3 antibody 10496-1-APDownload protocol
IF protocol for HOXD3 antibody 10496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols