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验证数据展示

  • IHC staining of human breast cancer using 10921-1-AP

    IHC staining of human breast cancer using 10921-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10921-1-AP (TAF9B antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HeLa using 10921-1-AP

    IP experiment of HeLa using 10921-1-AP

    IP result of anti-TAF9B (IP:10921-1-AP, 4ug; Detection:10921-1-AP 1:300) with HeLa cells lysate 4000ug.

TAF9B Polyclonal antibody

TAF9B Polyclonal Antibody for IHC, IP, ELISA
Cat No. 10921-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IP, ELISA

别名

TAFII31L, TAF9L, Neuronal cell death-related protein 7, DN-7, DN7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10921-1-AP targets TAF9B in IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TAF9B fusion protein Ag1362 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, 31kDa
别名 TAFII31L, TAF9L, Neuronal cell death-related protein 7, DN-7, DN7
计算分子量 28 kDa
观测分子量 26 kDa
GenBank蛋白编号BC010350
基因名称 TAF9B
Gene ID (NCBI) 51616
RRIDAB_2199344
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBM6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription initiation factor TFIID subunit 9B (TAF9B), also names neuronal cell death-related protein 7,transcription initiation factor TFIID subunit 9-like,transcription-associated factor TAFII31L. It is essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.It's molecular mass is 31kd.

实验方案

Product Specific Protocols
IHC protocol for TAF9B antibody 10921-1-APDownload protocol
IP protocol for TAF9B antibody 10921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols